Topics
Recombinant DNA Technology: Cutting & pasting of DNA molecules (i.e. restriction digestion, ligation, DNA gel eectrophoresis, and gel extraction.)
Bioinfo part: Bioinformatics of DNA database / Sequence
Analysis: Pairwise sequence alignment, Multiple sequence alignment, Pattern search.
Tools: BLAST, CLUSTALW/X, CLC Bio Main Workbench
Genetic Engineering: Transformation and plasmid purification, cloning and sub-cloning, amplification of DNA fragments by polymerase chain reaction (PCR).
Bioinfo part:
• Designing of PCR primers
• In-silico cloning
• Searching for homologous and paralogous sequences
Tools: CLC Bio Main Workbench, BLAST
Genomics Data Visualization: Browsing and comparing human and mouse genomic data with the help of NCBI Genome viewer and UCSC Genome browser.
Basic Biostatistics for Molecular Biologist:
• Data visualization and summarization techniques
• Univariate and Bivariate data analysis